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KMID : 0903520090520050448
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2009 Volume.52 No. 5 p.448 ~ p.455
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Flavonoids from the Leaves of Thuja orientalis Inhibit the Aldose Reductase and the Formation of Advanced Glycation Endproducts
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Lee Eun-Ha
Song Dae-Geun
Lee Joo-Young
Pan Cheol-Ho
Um Byung-Hun
Jung Sang-Hoon
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Abstract
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The purpose of this study is to evaluate the active properties of Thuja orientalis leaves for the treatment of diabetic complications. The ethyl acetate fraction showed a significantly higher inhibition both of recombinant human aldose reductase (rhALR2) inhibitory activity and advanced glycation endproducts (AGEs). The detected antioxidants (compounds 1-4) by online-HPLC-ABTS+ method and other three compounds were isolated using preparative RP-HPLC and Sephadex LH-20 column chromatography. Among the seven compounds, compound 4 (quercitrin) which was isolated from ethyl acetate fraction, was found to show inhibition for both forms of rhALR2 and AGEs. This compound also inhibited oxidative stress which was measured by Photochem® apparatus. In conclusion, the ethyl acetate fraction from T. orientalis demonstrated antioxidant activity as well as inhibitory effects on rhALR2 and AGEs. Quercitrin was shown to be the active compound and hence could be offered as an active material of standardization for the development of natural products for food or medicines.
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KEYWORD
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advanced glycation endproducts, aldose reductase, online-HPLC-ABTS+ method, quercitrin, Thuja orientalis
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